The urinary lead concentrations had been positively affiliated with each systolic and diastolic blood pressure level, but urinary selenium concentrations have been negatively connected to them.

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We requested the individuals to gather their place urine samples using a urine cup and dispensed the samples into screw-capped polypropylene tubes.

The tubes ended up washed with fifteen% nitric acid and rinsed with ultrapure drinking water just before sampling. The collected urine samples were frozen with prevmed joao pessoa dry ice, then transported to Japan, and saved at −eighty°C right up until Examination.

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